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Talk:Gel electrophoresis

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Umm, this is mostly about molecules, not about electrophoresis... -- Marj Tiefert as it should be - gel electrophoresis is used almost exclusively to seperte and characterize molecules; the theory is not of much intrinsic interest.


[edit] Gel electrophoresis =/ electrophoresis

This section should be deleted as the electrophoresis article makes all this moot USP<726> cites Gel, Disk, Zone among others. Also, USP<727> Capillary Electrophoresis, as well as Capillary Zone electrophoresis. Perhaps electrophoresis should not redirect here.

Eh, I'm an idiot, although I do still think Gel electrophoresis is still too specific for electrophoresis to redirect here.Ix 01:47, 31 May 2004 (UTC)


Its not very accurate either. Normally electrophoresis of marcomolecules separates based on size and shape, rather than charge, or at least that's the way it is used. You can also separate based upon the isoelectric point. I think it would be worthwhile redoing the article. This probably should be one place that electrophoresis is linked, but surely not the only one. srlasky 22:43, 2004 Oct 24 (UTC) Both are wrong. Electrophoresis and Gel electrophoresis seperate on a combination of NET charge and frictional resistance. The net charge of a molecule depends on the surrounding medium; the frictional resistance is mostly a function of shape - how hard it is for the molecule to flow through the gel. basically velocity = electrical force X net charge - friction. (in some cases, the two scale with size, this is why there is no size dependent seperation of dna without a gel).aditya

[edit] Electrophoresis redirect?

This section candidate for deletion now that electrophoresis article available Think electrophoresis should be seperate article. Electrophoresis refers to the movement of charge particles in a medium induced by an electric potential, and gel electrophoresis is merely the most commom application, strongly recommend that electrophoresis not redirect to this article. Does not adequately explain concepts such as the role of the zeta potential, sediment potentials, streaming potential, electrophoretic mobility etc, nor does it explain related phenomena such as electroosmosis and other electrokinetic actions. Also does not allow for proper understanding of equations modelling electrophoretic movement for example Smoluchowski equation and O'Brien and White's model.

Strongly believe electrophoresis should be a more general article linking to this page and other applications such as electrophoretic deposition.

Someone made electrophoresis a separate article recently, FYI. Nathan J. Yoder 22:52, 15 October 2005 (UTC)

[edit] A couple of suggestions

I think the first pragraph should give a hint of the modus operandi of the method, ie say that the separation occurs because of different velocity. I also think it would be more explanatory to say that one of the factors that determine the speed is the charge of the molecule, rather than say that it is the isoelectric point. The isoelectric point is just one of the factors that determine the charge that determines the velocity. The other factor is the pH of the medium in which it moves. The pH is normally determined by the buffer that is added. matrix is a word that is often used for the gel or medium in which the molecules move. But matrix is a word that has many meanings in different contexts. gel should be appropriate, or possibly medium. --Etxrge 13:15, 6 January 2006 (UTC)







"...applying an electric current, the molecules will move through the matrix at different rates, towards the anode if negatively charged or towards the cathode if positively charged (note that gel electrophoresis operates as an electrolytic cell; the anode is positive and the cathode is negative)."

Can this be elaborated on? I thought that electrons always ran from cathode to anode. But it seems like the terminology is reversed for electrophoresis.

Does the negatively charged DNA migrate in the same direction as the electrons?

Anode and Cathode are confusing terms. What is important is that the anode is positively charged [A good source]. The wiki article on cathode can help clear up some of the confusion.

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