Talk:RuBisCO
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Rubisco was a science collaboration of the week.
View the changes made during that week.
Since when has this enzyme been called rubisCO instead of rubisco? It was rubisco (or usually, its long name) when I was in grad school working on photosynthesis, and the website linked to from the article calls it rubisco. Can we change it back so that rubisco has the article and rubisCO is the redirect? -- Marj 18:28, 6 Oct 2004 (UTC)
I have moved the article to the short from of the name since that's what people call it in practice and since there is some technical issues with / in titles.--nixie 05:32, 11 August 2005 (UTC)
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[edit] Calvin Cycle diagram
Hey I just made/added an overview of the calvin cycle for this page and the calvin cycle page, I was wondering what everybody thinks. I'm worried it's to complicated/busy, I am wondering if i should dumb it down (ie get rid of the molecule diagrams). Anything else to change, I really want it to be great. Adenosine | Talk 08:34, September 2, 2005 (UTC)
- I think it is good to include such a diagram on this page. Please check the structure of 3-phosphoglycerate; I think you have a carbon with 5 bonds and an extra hydrogen. Is it reasonable to show the carboxyl group as protonated at physiological pH? I like the idea of science articles that start simple and then have the details towards the end. Maybe there could be a simplified figure right at the start of the article. --JWSchmidt 13:35, 2 September 2005 (UTC)
- Questions about Figure 2:
--JWSchmidt 20:41, 2 September 2005 (UTC)
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- You'd start to think I don't study this stuff every day in school... fixed. Adenosine | Talk 20:52, September 3, 2005 (UTC)
- I tried making a new version (Image:Calvin-cycle3.png) that emphasizes the labels for RuBisCO and "Phase 1". It allows my old eyes to better see "RuBisCO" in the thumbnail view. If you think the modified version is okay, it probably could be compressed to a smaller file size. --JWSchmidt 09:12, 5 September 2005 (UTC)
- You'd start to think I don't study this stuff every day in school... fixed. Adenosine | Talk 20:52, September 3, 2005 (UTC)
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[edit] Many changes
I'm trying to learn the editing procedures/protocols (so patience please) and I have started to make many changes to this entry to hopefully improve it, based on the latest literature and consensus views on this enzyme. More to come, including references to better document the changes I have made. Need to create a User name - ARP for now 130.126.53.185 23:49, 29 November 2005 (UTC)
- Thanks for all of your work on this article. --JWSchmidt 01:28, 30 November 2005 (UTC)
[edit] Added figure.
Added a figure showing the complete enzyme. I think this image should replace or at least accompany figure 1, but I'm just learning this whole system.
Made some minor changes in the text - links. Will add refs soon. ARP 17:16, 30 November 2005 (UTC)
- Feel free to change the image for Figure 1. Figure 1 resides withing a "protein information box". The information box is generated by a "template": Template:Protbox_start. You can edit the "Photo" and "Caption" parameters for the template where it is used right at the start of the RuBisCO edit window:
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- {{Protbox start|Name=[[RuBisCO]]|Photo=RuBisCO.jpg|Caption='''Figure 1'''. Charged domains [<span style="color:#ff0000;">red</span>(<big>-</big>), <span style="color:#0000ff;">blue</span>(+)] on [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?form=6&db=t&Dopt=s&uid=30476 RuBisCO] allow it to assemble into tightly packed multi-meric complexes, maximizing the number of copies of this inefficient enzyme inside plant cells.....
- Just replace "RuBisCO.jpg" with the name of a better image and add a descriptive caption for the new image. --JWSchmidt 19:47, 30 November 2005 (UTC)
[edit] Added references and some text changes
I'll have to mess around with replacing/switching Figs 1&4.ARP 21:59, 30 November 2005 (UTC)
[edit] Switched figures
OK, I've switched figs 1 and 4. Added one more ref to 'genetic engineering' section and made some small text changes. Also note that in Fig 2, Ribulose 1-phosphate (& its structure) should be replaced by Ribulose 5-phosphate - I've emailed the original author and hopefully he will change it.ARP 16:22, 1 December 2005 (UTC)
[edit] Switched figures
OK, I've switched figs 1 and 4. Added one more ref to 'genetic engineering' section and made some small text changes. Also note that in Fig 2, Ribulose 1-phosphate (& its structure) should be replaced by Ribulose 5-phosphate - I've emailed the original author and hopefully he will change it.ARP 16:22, 1 December 2005 (UTC)
[edit] rubiscos structure
Rubisco consists of eight large L chains (56 kd) and eight small S chains (14 kd) giving an L8S8 octo-dimer. NOT four L chains and eight S chains!
[edit] Metabolic control
The article states that "RuBisCO is the primary rate-limiting enzyme of the Calvin cycle." - NO NO NO! Under normal physiological conditions, according to Quick et al (1991), Rubisco has a control coefficient of 0.05-0.15 (the sum total of the control coefficients of every enzyme in a given pathway being 1). Only when growth has taken place in low light, and light intensity is suddenly increased (or CO2 is suddenly decreased), does rubisco's control coefficient increase to coefficient ranges where this statement might be accurate (>0.5).
Under the "normal" high light intensities typical of outdoors on sunny days, the rubisco control coefficient is 0.8 - see the paper following Quick (Stitt et al., 1991). Studies in the Quick paper were conducted using growth chamber conditions - low light!!!. Thus the statement is correct. ARP 19:22, 20 December 2006 (UTC)
[edit] Importance
Considering that RuBisCo makes up a majority of the protein on Earth I think that it should be upgraded to high/top level importance. Anyone else agree? —The preceding unsigned comment was added by R-Bowen J (talk • contribs) 08:11, 19 December 2006 (UTC).
- Even if it is the most common protein, that does not mean it "makes up a majority of the protein on Earth". --JWSchmidt 02:58, 1 February 2007 (UTC)