PBR322
From Wikipedia, the free encyclopedia
- The correct title of this article is pBR322. The initial letter is shown capitalized due to technical restrictions.
pBR322 is a plasmid and one of the most commonly used E. coli cloning vectors. pBR322 was the first artificial plasmid created by Bolivar and Rodriguez and was named eponymously after the scientist duo. pBR322 is 4361 base pairs[1] in length and contains a replicon region (source plasmid pMB1) and the tet gene, encoding the tetracycline resistance protein (source plasmid pSC101). It also contains the unique EcoRI restriction site GAATTC and splices the DNA between G and A from 5' to 3' position.
[edit] Trivia
In the Michael Crichton novel Jurassic Park, the included dinosaur DNA sequence is actually sequence from pBR322.
[edit] External links
- Overview
- PhD dissertation: Control of pBR322 plasmid replication in vivo, Chuen-Sheue Chiang, The University of Texas at Dallas
- Explanation of splice
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[edit] References
1) Watson, N., A new revision of the sequence of plasmid pBR322, Gene 70, 399-403, 1988.
