Afbeelding:Pcr.png
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Pcr.png (552 × 555 pixels, bestandsgrootte: 4 KB, MIME-type: image/png)
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Nederlands: Schematische voorstelling van de PCR ketingreactie.
1. De denaturatie van de de dubbele helix naar een enkele DNA-streng bij 96 °C.
2. hybridizatie van de enkelstreng met primers bij 68 °C.
3. vervollediging van de DNA-enkelstrengen bij 72 °C (p= polymerase).
4. Eind van de eerste cyclus.
in de praktijk wordt deze cyclus vele malen herhaald.
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[edit] inaccuracy in diagram
This picture is not accurate, as it depicts reannealing of strands (eg the original blue strands anneal back together). In PCR, any partner strand is always the newly extended primer from the last extension phase, it doesn't reanneal with an old or different partner strand. -Madeleine
- Description:
-
English: Schematic drawing of the PCR cycle.
- Denaturing at 96°C.
- Annealing at 68°C.
- Elongation at 72°C (P=Polymerase).
- The first cycle is complete. The two resulting DNA strands make up the template DNA for the next cycle, thus doubling the amount of DNA duplicated for each new cycle.
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Deutsch: Schematische Darstellung des PCR-Zyklus.
- Denaturalisieren/Schmelzen bei 96 °C.
- Anlagerung bei 68 °C.
- Verlängerung bei 72 °C (P=Polymerase).
- Der erste Zyklus ist beendet.
- Source: From English Wikipedia, uploaded on 27th February 2003 by Magnus Manske with original description »From Nupedia«
* (Delete all revisions of this file) (cur) 03:34, 26 November 2005 . . R. Koot (Talk) . . 688x695 (49,973 bytes) (Created by User:Magnus Manske and donated to Nupedia.) * (del) (rev) 03:24, 26 November 2005 . . R. Koot (Talk) . . 552x555 (4,427 bytes) (Reverted to earlier revision) * (del) (rev) 03:18, 26 November 2005 . . R. Koot (Talk) . . 688x695 (46,904 bytes) (The 3\\'s and 5\\'s mark the orientation of the DNA strands. Many enzymes run only in one direction along a DNA strand. For example, DNA-Polymerase runs toward the 3\\' end. Created by User:Magnus Manske and contributed to Nupedi) * (del) (rev) 22:53, 27 February 2003 . . Magnus Manske (Talk) . . 552x555 (4,427 bytes) (From Nupedia)
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