Image:ELISA-sandwich.svg

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Description	A sandwich ELISA. (1) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added, and binds to antigen; (4) enzyme-linked secondary antibody is added, and binds to detecting antibody; (5) substrate is added, and is converted by enzyme to detectable form.
Source	Own work
Date	Designed 2006-05-08
Author	Jeffrey M. Vinocur
Permission	Own work, attribution required (Multi-license with GFDL and Creative Commons CC-BY 2.5)

I, the author of this work, hereby publish it under the following licenses:

Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled "GNU Free Documentation License".

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ELISA

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Image:ELISA-sandwich.svg

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