Acridine orange
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| Acridine orange | ||
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| General | ||
| Systematic name | N,N,N',N'-tetramethylacridine- 3,6-diamine |
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| Other names | Euchrysine 3,6-Acridinediamine Waxoline Orange A Acridine Orange Base Solvent Orange 15 Rhoduline Orange Rhoduline Orange N Acridine Orange NO Rhoduline Orange NO Basic Orange 14 |
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| Molecular formula | C17H19N3 | |
| SMILES | CN(C)C1=CC2=C(C=C1)C= C3C=CC(=CC3=N2)N(C)C |
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| Molar mass | 265.35 g/mol | |
| Appearance | Orange powder | |
| CAS number | 10127-02-3 | |
| Hazards | ||
| MSDS | External MSDS | |
| EU classification |  Xi N |
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| NFPA 704 |
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| R-phrases | R20/21/22 R40 | |
| S-phrases | S26 S28 S37 S45 | |
| RTECS number | AR7601000 | |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
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Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). The dye is often used in epifluorescence microscopy.
Acridine orange is prepared from coal tar and creosote oil.
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