Fast protein liquid chromatography
From Wikipedia, the free encyclopedia
Fast Protein Liquid Chromatography, usually referred to as FPLC, is a form of column chromatography used to separate or purify proteins from complex mixtures. It is very commonly used in biochemistry and enzymology. Columns used with an FPLC can separate macromolecules based on size, charge distribution, hydrophobicity, or biorecognition (as with affinity chromatography).
[edit] Trademark
- FPLC is a trademark of GE Healthcare, formerly Pharmacia Biotech.
[edit] Typical columns
Typical columns used for protein purification include:
- Ion exchange chromatography, cation or anion — separate proteins based on surface-charges
- Gel filtration chromatography and Gel permeation chromatography — separate proteins based on size.
- Reversed phase or hydrophobic interaction — separates based on hydrophobicity which depends heavily on surface area
- Affinity chromatography — columns which purify based on ligand affinity, such as a His-tagged protein would use a nickel column.
[edit] See also
- Chromatography, an overview article covering all chromatographic techniques.
- High performance liquid chromatography (HPLC) for column chromatography using high pressure.
- Affinity chromatography
