Talk:Heat shock protein
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BROKEN LINK: the link to the faculty member's website is broken...
This page is merged-from Heat-shock protein. See its history in the redirect, if you wish.
The two pages had different POV's. Both assume the cells survive the heat shock.
The current lead page paragraph has the idea that genetic factors influence the expression of the HSPs. Thus there is a molecular slant to the POV. The transcriptional genetics POV needs some references to disentangle whether there is an actual molecular process which creates the HSPs. If that is so, then HSP production could be performed via the recombinant DNA technology. Need references.
The H-SP page had the idea that structural factors (or regulatory factors) influence cellular and organ development. Thus there is the concept that heat stress somehow banks the H-SPs, in larger and larger amounts, without destroying the cell itself, which is a factor in the cellular or organ survival. If an HSP is like a hormone, then we need references to show the influence/quantity needed for development of some cellular or organ structure. If the growth in amount of H-SP shows grains or chains of H-SP in the cell itself, then that would be an argument for a simple manufacturing process (due to the stress) within the cell, without recourse to RNA or DNA mechanisms. Again, we need references. -Problems and criticism- I need to return to this page to somehow integrate the two separate streams of thought. It helps to have them on the same page. Ancheta Wis 08:49, 13 Jan 2005 (UTC)
Original contributors: please respond.
Hi,
The heat shock proteins are indeed regulated at the transcriptional level. The specific transcription factor has been identified; in humans the key one is HSF1. A specific heat shock promoter region has also been identified, to which HSF1 binds. The heat shock promoter is located just upstream of all the heat shock protein genes. In response to stress, HSF1 is phosphorylated and trimerizes; the kinase that phosphorylates it is unknown, as is the particular signal that starts the process. That the heat shock response is transcriptionally regulated is also supported by the time it takes to see an increase in heat shock protein levels following cellular stress--more than half an hour, and increasing over the course of several hours.
Heat shock proteins don't seem to be toxic in most cells, even at relatively high levels. They actually tend to have antiapoptotic effects, both by acting as chaperones for the protein folding process, and by sequestering pro-apoptotic factors. Malignant cells often constitutively express high levels of some heat shock proteins (including at least Hsp70 and Hsp27; possibly others), possibly because a) it helps them to survive in the unpleasant tumour microenvironment, and b) it staves off any residual apoptotic machinery that they have remaining.
Note that heat shock proteins outside of cells can act as inflammatory signals. (The mechanism by which the inflammatory response is activated here is not well understood.) In general, HSPs are intracellular proteins only; their presence in the extracellular space is the likely result of necrosis or cell lysis and acts as a signal for that reason.
Actually, some function extracellulary related to platelet aggregation, cellular wound healing, and modulating immune responses GetAgrippa 04:59, 2 September 2006 (UTC)
Several members of the HSP family are regularly produced in the lab using conventional recombinant techniques. Stressgen Bioreagents sells them commercially for research purposes. [[1]]
There's a bit of a start, anyway. I must be off to work.... --TenOfAllTrades 13:50, 13 Jan 2005 (UTC)
[edit] Criticisms
I think the Researcher section is anectdotal and borders on advertisment. There are numerous researchers pursuing this subject. I find it offensive to the vast majority who are not mentioned.GetAgrippa 12:44, 31 August 2006 (UTC)
- I agree that they should be removed unless there is solid proof that these are the world's biggest experts on HSPs. JFW | T@lk 21:36, 31 August 2006 (UTC)
Jfdwolff, Does this sort of thing fall into vanity guidelines clause??GetAgrippa 04:44, 2 September 2006 (UTC)
Perhaps a history secton and mention notable names in various fields. Dr. Lundquist is a recognizable name, but others come immediately to my mind (that is subjective admittedly).GetAgrippa 11:47, 1 September 2006 (UTC)
The heat shock proteins have been linked to development, immunology, cancer, cardiovascular, and numerous other fields. This topic needs major expansion.GetAgrippa 12:44, 31 August 2006 (UTC)
Since the heat shocks are ancient molecules that are highly convserved ,perhaps it should be introduced with Groel and its chaperonin function. Then build on the theme with mammalian heat shock proteins and their role in numerous areas. The heat shock induction is of interest, but the endogeneous pool that serves in homeostasis perhaps is more significant.GetAgrippa 13:41, 31 August 2006 (UTC)
I just noticed the chaperonin link. Maybe start with brief histoy in bacteria and link to chaperonin, and then develop from there?GetAgrippa 11:49, 1 September 2006 (UTC)
[edit] Cardiovasculature Roles
I will organize and reference this section. Perhaps we should have sections for Cardiovascular, Immunology, Development, Cancer, etc. and link them to other articles?GetAgrippa 11:47, 1 September 2006 (UTC)
