Talk:Hepatocyte
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Isolated hepatocytes represent one of the most useful in vitro experimental systems for drug discovery and development. As the liver is the major organ for drug metabolism and often a target of drug toxicity, isolated hepatocytes can be used for the evaluation of metabolic stability, metabolite identification, pharmacokinetic drug-drug interactions, and drug toxicity. Our laboratory (Li, AP et al) has over 25 years of experience in hepatocyte isolation, culturing and cryopreservation. Our most recent accomplishment is the cryopreservation of human hepatocytes to retain high viability (routinely over 90% based on trypan blue exclucion) and attachment efficiency (over 50% of our cryopreserved lots can attach and form confluent monolayer cultures after thawing). We are now working to extend this cryopreservation technique to animal hepatocytes (we will start with rat hepatocytes). I would like to welcome discussion on isolated hepatocytes from the public, especially on new science, techniques and applications.Lialbertpakhung 11:59, 24 February 2007 (UTC)
