Neutral red
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| Neutral red | |
|---|---|
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| Systematic name | toluylene red |
| Chemical formula | C15H17ClN4 |
| Molecular mass | 288.78 g/mol |
| Density | x.xxx g/cm3 |
| Melting point | 290°C |
| Boiling point | °C |
| CAS number | [553-24-2] |
| SMILES | CC3=CC2=NC1=CC=C (N(C)C)C=C1N C2=C/C3=[N+](C)/C |
| Disclaimer and references | |
| Neutral red (pH indicator) | ||
| below pH 6.8 | above pH 8.0 | |
| 6.8 | ↔ | 8.0 |
Neutral Red (or toluylene red, Basic Red 5, or C.I. 50040) is an eurhodin dye used for staining in histology. It stains cell nuclei red. It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods. Together with Janus Green B it is used to stain embryonal tissues and supravital staining of blood. Can be used for staining Golgi apparatus in cells and Nissl granules in neurons.
Neutral red can be used as a vital stain, to stain living cells. It is used to stain cell cultures for plaque titration of viruses.
Neutral Red is added to some growth media for bacteria and cell cultures. It usually comes as a chloride salt.
Neutral Red acts as a pH indicator, changing from red to yellow between the pH 6.8-8.0.
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