Talk:Proteome
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[edit] X-ray spectrometry
Does anyone else feel that x-ray spectroscopy should be mentioned? I realize this isn't terribly popular right now due to the massive expense of equipment, but there is exciting progress being made. And it reveals more than mass spectroscopy, especially regarding structure. I think it'll be a big deal for proteomics, heme research has already seen significant gains.
[edit] Cell proteome vs organism proteome
The following is from the Proteomics page:
"The entirety of proteins in existence in an organism throughout its life cycle, or on a smaller scale the entirety of proteins found in a particular cell type under a particular type of stimulation, are referred to as the proteome of the organism or cell type respectively."
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I think the term "proteome" is in need of some conceptual clarification. It can make sense to tie the term to individual cells. It is in the context of a functioning network of transcription factors inside a cell that we can begin to make sense of why a particular set of genes will be expressed.
Science, Vol 270, Issue 5235, 369-370 , 20 October 1995 "Mission Impossible: Follow the changes taking place in a cell by identifying the thousands of different proteins the cell produces and watching how they ebb and flow over time. But a growing band of researchers believes that improved analytical techniques may soon make such an approach feasible, and they have even coined a new term for it: proteome research."
But it also makes some sense to conceptualize the sum total of all the individual cellular proteomes in an entire multi-cellular organism.
Biotechnol Genet Eng Rev. 1996;13:19-50. Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it.
Science, Vol 291, Issue 5507, 1221-1224 , 16 February 2001 "A single gene can encode multiple different proteins--these can be produced by alternative splicing of the mRNA transcript, by varying translation start or stop sites, or by frameshifting during which a different set of triplet codons in the mRNA is translated. All of these possibilities result in a proteome estimated to be an order of magnitude more complex than the genome."
The term "proteome" has also been used in the context of subcellular bilogical systems such as viruses.
J Proteome Res. 2002 Nov-Dec;1(6):501-13. "Analysis of the adenovirus type 5 proteome by liquid chromatography and tandem mass spectrometry methods. JWSchmidt 22:10, 28 Mar 2004 (UTC)
