Scatchard equation
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The Scatchard equation is an equation for calculating the affinity constant of a ligand with a protein. The Scatchard equation is given by (r/c) = Ka*n - Ka*r, where r is the ratio of the concentration of bound ligand to total available binding sites, c is the concentration of free ligand, and n is the number of binding sites per antibody molecule.
Ka is the association (affinity) constant from the equation Ka = [Ab-Ag]/([Ab][Ag]), where Ab is the binding site on the antibody, Ag is a monovalent antigen, and Ag-Ab is antigen-bound antibody.
The Scatchard equation is sometimes referred to as the Rosenthal-Scatchard equation.
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