Filter fluorometer
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A filter fluorometer is a type of fluorometer that may be employed in fluorescence spectroscopy.
[edit] Filters
There are two filters for the fluorometer:
- The primary filter or excitation filter or incident light filter isolates the wavelength that will cause the compound to fluoresce (the incident light).
- The secondary filter isolates the desired emitted light (fluorescent light).
[edit] Choosing the correct filters
The proper selection of filters requires familiarity with the emission spectrum and the excitation spectrum. The primary filter is selected to transmit only the wavelengths from the emission spectrum and excitation spectrum that overlap.
[edit] Types
- Turner 110
There area number of filter fluorometer types. They range from Plant Science based, which are used extensively to detect, measure and analyse fluorescence from chlorophyll during the photosynthesis process, to general purpose fluorometers tuned to the specific fluorescence porperties of target samples. Most common targets are Chlorophyll, Fluorescein, Rhodamine, and Green Fluorescent Protein. Customized dyes and taggants have been developed for a wide variety of applications including; waste water tracking, biologic activity, part inpsection, conterfeit detection, genetic tracing, disease and health studies, and DNA replication.
Additional types include Opti-Sciences' GFP-Meter, GFL-1, and a variety of OEM designs.
Turner 110 is manufactured by Turner Designs GFP & GFL-1 are manufactured by Opti-Scineces, Inc.
