Lowry protein assay
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The Lowry protein assay, or Lowry Method, is a biochemical test to measure the amount of protein in a sample.
Copper(II) ion in alkaline solution reacts with protein to form complexes, which react with the Folin-phenol reagent, a mixture of phosphotungstic acid and phosphomolybdic acid in phenol. The product becomes reduced to molybdenum/tungsten blue and can be detected colorimetrically by absorbance at 650 nm.
Presence of strong acids or ammonium sulfate can interfere with the assay.
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Experimental : Protein purification | Green fluorescent protein | Western blot | Protein immunostaining | Protein sequencing | Gel electrophoresis/Protein electrophoresis | Protein immunoprecipitation | Peptide mass fingerprinting
Bioinformatics : Protein structure prediction | Protein-protein docking | Protein structural alignment | Protein ontology | Protein-protein interaction prediction
The final blue colored mixture is usually read at 750nm.
