Nitro blue tetrazolium chloride
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| Nitro blue tetrazolium chloride | |
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| Systematic name | Nitro blue tetrazolium chloride |
| Chemical formula | C40H30Cl2N10O6 |
| Molecular mass | 817.64 g/mol |
| Density | x.xxx g/cm³ |
| Melting point | xx.x °C |
| Boiling point | xx.x °C |
| CAS number | [xx-xx-xx] |
| SMILES | [Cl-].[Cl-].[O-][N+](=O)c1ccc(cc1)n2nc (n[n+]2c3ccc(cc3OC)c7ccc([n+]5nc (nn5c4ccc(cc4)[N+]([O-])=O) c6ccccc6)c(OC)c7)c8ccccc8 |
| Disclaimer and references | |
Nitro blue tetrazolium (NBT) is a chemical.
- Color: yellowish.
- Solubility: very little solubility in water or lipid.
reduction
NBT (yellowish) → → blue precipitate
It is used in immunology for sensitive detection of alkaline phosphatase (with BCIP). NBT serves as the oxidant and BCIP is the AP-substrate (and gives also dark blue dye). It is also used as an electron acceptor for colorimetric/spectrophotometric activity assays of oxireductases (such as the mitochondrial electron transport chain complexes). Also used as a diagnostic test for Chronic Granulomatous Disease. A disease where there is a defect in NADP oxidase, therefore the phagocyte is unable to make the reactive oxygen species or radicals required for bacterial killing and results in bacteria thriving within the phagocyte. Treatment is by administering aggressive antimicrobial therapy able to kill bacteria intracellularly like Co-trimoxazol
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