Orange G

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Orange G, Acid Orange 10, or C.I. 16230, is a synthetic azo dye used in histology in many staining formulations. It usually comes as a disodium salt. It has the appearance of orange crystals or powder.

The main use of Orange G is in the OG6 Papanicolaou stain, to stain keratin.

It is often combined with other yellow dyes and used to stain erythrocytes in the trichrome methods.

Orange G can be used as a color marker to monitor the process of agarose gel electrophoresis, running approximately at the size of a 50 Base pair (bp) DNA molecule, and polyacrylamide gel electrophoresis. Bromophenol blue and xylene cyanol can also be used for this purpose.

Its CAS number is [1936-15-8] and its SMILES structure is OC1=C(N=NC3=C C=CC=C3)C2=C (C=C(S(=O)([O-]) =O)C=C2S(=O) ([O-])=O)C=C1. Its risk and safety phrases are R36/37/38 S26 S36.