Phalloidin
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Phalloidin is a toxin from the death cap (Amanita phalloides) that binds actin, preventing its depolymerization and poisoning the cell. Phalloidin binds specifically at the interface between F-actin subunits, locking adjacent subunits together. Phalloidin, a bicyclic heptapeptide, binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, which essentially stabilizes actin filaments through the prevention of filament depolymerization.[1] Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin.[2] Thus, phalloidin traps actin monomers in a conformation distinct from G-actin and it stabilizes the structure of F-actin by greatly reducing the rate constant for monomer dissociation, an event associated with the trapping of ADP.[2] Overall, phalloidin is found to react stoichiometrically with actin, strongly promote actin polymerization, and stabilize actin polymers.[3]
Shown above is the chemical structure of the phalloidin, a toxin from the “death cap” or “angel of death” mushroom, Amanita phalloides. This image was taken from the Cell Motility and The Cytoskeleton page: http://www.bio.umass.edu/vidali/web/cell_motil/phalloidin.gif, accessed 060218
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[edit] Function
Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin as well as filamin into stable “islands” of aggregated actin polymers, yet it does not interfere with stress fibers, i.e. thick bundles of microfilaments.[3]Wehland et al. also notes that at higher concentrations, phalloidin induces cellular contraction.
[edit] Phalloidin as an Imaging Tool
The property of phalloidin is a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro.[1] A high-resolution technique was developed to detect F-actin at the light and electron microscopic levels by using phalloidin conjugated to the fluorophore eosin which acts as the fluorescent tag.[4] In this method known as fluorescence photo-oxidation, fluorescent molecules can be utilized to drive the oxidation of diaminobenzidine (DAB) to create a reaction product that can be rendered electron dense and detectable by light microscopy.[4] The amount of fluorescence visualized can be used as a quantitative measure of the amount of filamentous actin there is in cells if saturating quantities of fluorescent phalloidin are used.[1] Consequently, immunofluorescence microscopy along with microinjection of phalloidin can be used to evaluate the direct and indirect functions of cytoplasmic actin in its different stages of polymer formation (Wehland et al., 1977). Therefore, fluorescent phalloidin can be used as an important tool in the study of actin networks at high resolution.
[edit] Limitations of Phalloidin
Phalloidins do not permeate cell membranes, making them less effective in experiments with living cells. Cells treated with phalloidins exhibit a number of toxic effects and frequently die.[1] Furthermore, it is important to note that phalloidin-treated cells will have greater levels of actin associated with their plasma membranes, and the microinjection of phalloidin into living cells will change actin distribution as well as cell motility.[1]
[edit] Background
Pioneering work on this toxin was done by the Nobel laureate Heinrich Wieland in the 1930's. Phalloidin was ultimately purified and crystallized in 1940 by Heinrich's student and son-in-law Feodor Lynen (who won a Nobel Prize in 1964 for his work on cholesterol metabolism), and Heinrich's nephew Ulrich Weiland.
[edit] See also
[edit] References
- ^ a b c d e Cooper JA (1987) Effects of Cytochalasin and Phalloidin on Actin. J. Cell Biol. 105 (4): 1473-1478. [1], accessed 060218
- ^ a b Barden JA, Miki M, Hambly BD and Dosremedios CG (1987) Localization of the phalloidin and nucleotide-binding sites on actin. Eur. J. Biochem. 162 (3): 583-588. [2], accessed 060218
- ^ a b Wehland J, Osborn M, and Weber K (1977) Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth. Proc. Natl. Acad. Sci. 74(12): 5613–5617. [3], accessed 060218
- ^ a b Capani F, Deerinck TJ, Ellisman MH, Bushong E, Bobik M and Martone ME(2001) Phalloidin–Eosin Followed by Photo-oxidation: A Novel Method for Localizing F-Actin at the Light and Electron Microscopic Levels. J Histochem Cytochem. 49(11): 1351-1362. [4], accessed 060218
