DCPIP
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DCPIP (2,6-dichlorophenolindophenol) is a blue chemical used as a dye as when it is in its oxidized state it is blue. Once reduced (by addition of electrons) it becomes colourless. This reaction can be reversed, and the colourless DCPIP will turn blue again, when it is oxidized. DCPIP is often used in measurements of the electron transport chain in plants as it has a higher redox potential (electron affinity) than the Fe-S complex in cytochrome b and will accordingly "steal" electrons from it, preventing cytochrome c from recieving them and cutting off photosystem I its electron supply. A common way of illustrating this change is by the addition of vitamin C solution, which reduces the 'jelly' transforming it to a colourless mass. This experiment can be carried out to illustrate the affect of changing cell sizes on SA/V (surface area:volume ratio).
[edit] References
- Mathews, Ahern, Van Holde, Biochemistry, Addison Wesley Longman Inc., 2000 pp534-5
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