Talk:Taq polymerase
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Quote: One of Taq polymerases' major drawbacks is its low replication fidelity. Without a 3' to 5' exonuclease proofreading mechanism to replace an accidental mismatch in the newly synthesized DNA strand, Taq polymerase cannot be used in experiments where an identical genetic sequence is required (such as in molecular cloning). End of quote
Actually Taq DNA polymerase can be used for cloning, usually it is just not worth the trouble and costs to use Pfu instead and one has to do a DNA sequencing of the vector plus DNA insert anyway. In all my dozens of cloning experiments I never had any trouble using Taq and never detected errors eventhough Taq has an error rate of 1 in 10,000.
Ph. D. in molecular biology
