Talk:Immunohistochemistry
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Ok it decribe the procces but what is measured? seen? and what is its goal?--Scubafish 18:46, 12 June 2006 (UTC)
Immunohistochemistry is a technique used by life scientists and sometimes pathologists to identify and localize substances of interest (e.g. infectious agents, tissue components) in light microscopical specimens. This is achieved by applying antibodies that are specific for the substance and that will exclusively bind to that substance and to nothing else. These antibodies as such are not visible, but by using 'labels' such as a dye, or a fluorescent chemical group, an enzyme or a colloidal metal (mostly gold) particle, these antibodies can be traced in the light microscope. Life scientists use the technique to explore where certain molecules occur in microscopical specimens so they may gain a better understanding of the funcion and location of these compounds. Pathologists occasionally use the technique as a means to identify diseased tissue, which helps in making decisions about medical treatment. The technique is also used in electron microscopy when a far more precise localization needs to be achieved. Because electron microscopy is more focused on the cellular and subcellular level than on tissue, the technique is called immunocytochemistry. Colloidal gold particles are used as labels.--222.152.190.188 03:46, 6 July 2006 (UTC)
[edit] Proposed_Merge_with_Immunohistochemical staining
The article are almost exactly the same. Need to be merged, both articles would benefit.--DO11.10 18:48, 16 March 2007 (UTC)
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- Agree I didn't notice that immunohistochemical staining existed as a page. It is redundant and not as thorough as immunohistochemistry. Merge ASAP Dr Aaron 06:22, 17 March 2007 (UTC)