Murashige and Skoog medium
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Murashige and Skoog medium or (MSO or MS0 (MS-zero)) is a plant growth medium used in the laboratories for cultivation of plant cell culture. MSO was invented by plant scientists Toshio Murashige and Folke K Skoog during Murashige's search for a new plant growth regulator.
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[edit] Ingredients
[edit] Macronutrients
- Ammonium nitrate (NH4NO3) 1,650 mg/l
- Boric acid (H3BO3) 6.2 mg/l
- Calcium chloride (CaCl2 · H2O) 440 mg/l
- Cobalt chloride (CoCl2 · 6H2O) 0.025 mg/l
- Magnesium sulfate (MgSO4 · 7H2O) 370 mg/l
- Cupric sulfate (CuSO4 · 5H2O) 0.025 mg/l
- Potassium phosphate (KH2PO4) 170 mg/l
- Ferrous sulfate (FeSO4 · 7H2O) 27.8 mg/l
- Potassium nitrate (KNO3) 1,900 mg/l
- Manganese sulfate (MnSO4 · 4H2O) 22.3 mg/l
- Potassium iodine (KI) 0.83 mg/l
- Sodium molybdate (Na2MoO4 · 2H2O) 0.25 mg/l
- Zinc sulfate (ZnSO4 · 7H2O) 8.6 mg/l
- Na2EDTA · 2H2Oa 37.2 mg/lb
[edit] Common organic additives
- i-Inositol 100 mg/l
- Niacin 0.5 mg/l
- Pyridoxine · HCl 0.5 mg/l
- Thiamine · HCl 0.1 mg/l
- IAA 1–30 mg/l
- Kinetin 0.04–10 mg/l
- Glycine (recrystallized) 2.0 g/l
- Edamine 1.0 g/l
- Sucrose 20 g/l
- Agar 10 g/l
[edit] References
- Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15(3): 473-497.
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