Talk:Ubiquitin

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Question - Is there a tumor with NF-KB and Ubiquitin affected?

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Could someone create / find a nice image of this protein? — David Remahl 20:35, 6 Oct 2004 (UTC)

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Does it have the same sequence in all eukaryotes? AxelBoldt 02:04, 7 Oct 2004 (UTC)

Is it really 'ubiquitous', as its name implies? ~GMH 05:23, 17 August 2005 (UTC)

I believe that is where the name comes from originally. It is present pretty much everywhere in eukaryotic cells. It is probably as important (if not more important) than phosphorylation in a massive variety of cellular function. "Darwin's Phosphate" has been used to describe it by our lecturer (R.J Mayer who edited the book cited by this article...he likes it a lot). --Sinkingpie 21:09, 14 May 2006 (UTC)

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Should we be using the term "ubiquitylation"? Is it not generally called "ubiquitination" at present? Jamie 17:20, 29 January 2006 (UTC)

I've rewritten the intro to reflect this. -- stillnotelf is invisible 21:21, 31 March 2006 (UTC)

Ubiquitination is in common usage even within the scientifc literature, although ubiquitylation is strictly speaking the correct terminology -- RogerDodger 22:41, 5 April 2006 (UTC)

Contents 1 Limpets for ubiquitin antibody? 2 Ubiquitin in Archaea and the Proteasome 3 VHL 4 Human ubiquitin sequence

[edit] Limpets for ubiquitin antibody?

Why are just keyhole limpets used for making ubiquitin antibody? Links for example: [[1]], [[2]]. --Snek01 14:19, 5 April 2006 (UTC)

Keyhole limpet haemocyanin is a commonly used immune stimulant - it is attached to the protein/peptide of interest and injected into an animal which then raises an antibody response against the protein.--RogerDodger 22:41, 5 April 2006 (UTC)

[edit] Ubiquitin in Archaea and the Proteasome

Should we have some mention of ubiquitin in archaebacteria? The protein is relatveily conserved from them to eukaryotes, but the article suggests that it is only important in the latter.

There also seems to be a large emphasis on the proteasome. I think that thought is generally moving away from ubiquitin being simply a signal for degradation. e.g. transcriptional regulation, DNA repair etc. (the book mentioned in further reading has a lot on this sort of stuff). --Sinkingpie 10:20, 10 May 2006 (UTC)

I added a mention of mono-ubiquitination to deal with this point... though did not use your examples, so you may want to add those. Gacggt 04:49, 27 May 2006 (UTC)

[edit] VHL

VHL is a member of a complex with E3 ubiquitin ligase activity. VHL itself is not an E3 ubiquitin ligase.

[edit] Human ubiquitin sequence

The human ubiquitin sequence is: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

This section was added by anonymous user 86.6.171.123 on December 18, 2005. Can someone verify what this is, and perhaps explain to a layman what this means, either with a wikilink or a couple of sentences to assure us that it isn't just someone pounding on their keyboard? Neil916 (Talk) 18:47, 4 January 2007 (UTC)

This is the amino acid sequence of the mature Ubiquitin. Each one of these letter represents one amino acid. All is legit. Here is the NCBI page for the Ubiquitin precursor that results from the translation of the human UBB (one of the few genes that code for ubiquitin) mRNA - scroll at the very bottom of it where you'll find a small section called "ORIGIN"

This is the amino acid sequence of the ubiquitin precursor. I have colored the part that is going to be mature ubiquitine, after the precursor gets cleaved, and as you can see it is identical to the one in the article.

If you go to the NCBI protein page of another human gene that codes for ubiquitin - UBC - you'll find the same sequence embedded in a different precursor.

I hope that makes it clear for ya. -- Boris 10:31, 11 January 2007 (UTC)